PAINE #PANCE Pearl – Pediatrics



What are the organisms that you must think of in a sick neonate that can be transmitted in utero from mother to fetus?



There are 10 organisms that are most commonly transmitted in utero from mother to fetus and are remembered by the mnemonic: TORCHESCLAP




  1. Tagg A. Passing the TORCH. Don’t Forget The Bubbles Blog.


Aicardi Syndrome

DefinitionCongenital syndrome with three main features:

  1. Agenesis or dysgenesis of the corpus callosum
  2. Infantile spasms and/or epilepsy
  3. Chorioretinal lacunae

Clinical Significance Occurs almost exclusively in females and clinical findings can include:

  • Asymmetry of cerebrum
  • Ventricular cysts
  • Microcephaly
  • Severe developmental delay and disability
  • Ocular abnormalities (microphthalmia, colobomo)
  • Short philtrum with flat nose and upturned ears
  • Sparse eyebrows
  • Small hands
  • Spinal abnormalities

History – Named after Dr. Jean Fraçois Marie Aicardi, who is a French pediatrician, and first published and described this disorder in two girls in 1965.


  1. Firkin BG and Whitwirth JA.  Dictionary of Medical Eponyms. 2nd ed.  New York, NY; Parthenon Publishing Group. 1996.
  2. Bartolucci S, Forbis P.  Stedman’s Medical Eponyms.  2nd ed.  Baltimore, MD; LWW.  2005.
  3. Yee AJ, Pfiffner P. (2012).  Medical Eponyms (Version 1.4.2) [Mobile Application Software].  Retrieved
  4. Whonamedit – dictionary of medical eponyms.
  5. J. Aicardi, J. Lefebvre, A. Lerique-Koechlin. A new syndrome: Spasm in flexion, callosal agenesis, ocular abnormalities. Electroencephalography and Clinical Neurophysiology. 1965;19:609-610.


Pemberton’s Sign

DefinitionRaising of the patient’s arms over their head (until the arms touch their face) causes flushing and congestion of head and neck due to venous congestion and thoracic inlet obstruction.

Image result for pemberton sign

Clinical Significance Indicates superior vena cava syndrome in patients with mediastinal mass

History – Ok…so I have conflicting sources on this one:

  1. Dr. Hugh Spear Pemberton (1890-1956) – English physician who first described it in a letter to The Lancet in 1946.
  2. Dr. John de J. Pemberton (1887-1967) – US surgeon who was a pioneer in thyroid surgery


You make the call:

Image result for english flag     or     Image result for us flag


  1. Firkin BG and Whitwirth JA.  Dictionary of Medical Eponyms. 2nd ed.  New York, NY; Parthenon Publishing Group. 1996.
  2. Bartolucci S, Forbis P.  Stedman’s Medical Eponyms.  2nd ed.  Baltimore, MD; LWW.  2005.
  3. Yee AJ, Pfiffner P. (2012).  Medical Eponyms (Version 1.4.2) [Mobile Application Software].  Retrieved
  4. Whonamedit – dictionary of medical eponyms. Accessed March 7, 2017.
  5. De Filippis EA, Sabet A, Sun MR, Garber JR. Pemberton’s sign: explained nearly 70 years later. The Journal of clinical endocrinology and metabolism. 2014;99(6):1949-54. [pubmed]
  6. Pemberton HS.  Sign of submerged goitre.  Lancet.  1946;248(6423):509.

#29 – Review of Transfusion Medicine with Michelle Brown





Michelle Brown, MS, MLS(ASCP)SBB

Clinical Education Coordinator, Assistant Professor

University of Alabama at Birmingham

Clinical Laboratory Science Program

Transfusion Statistics

According to the American Red Cross:

  • Someone in the US needs blood every 2 seconds
  • 36,000 units PRBC, 10,000 units of FFP, and 7,000 units of platelets are transfused every day in the US
  • Over 21 million blood components are transfused each year in the US, but only 13.6 million are collected

Process of Separation

When a person donates blood, 1 pint of whole blood is removed and then needs to be separated into its components for longer shelf life and targeted treatments.  There are 2 processes on how this occurs:

  • Platelet-Rich Process (PRP)
    • Easier, cheaper, but plasma/platelet yield is lower
    • BC Method
      • Complicated, but higher yield of plasma/platelet
  • Once the separation occurs, then the components can be treated with additives to help with viability and longevity. In the case of FFP and cryo, it is frozen to maintain potency of coagulation factors.

Packed Red Blood Cells

  • Facts
    • One unit of PRBC = 300cc
    • Hematocrit = 55-80%
    • One of PRBC should raise hemoglobin by 1g/dL and hematocrit by 3%
  • Storage Considerations
    • Can be stored for up to 42 days
    • Treated with citrate to prevent clotting
  • Indications for Transfusion
    • Recommendations from 2016 AABB Guidelines
      • ***To pair with patient symptoms***
      • Hemodynamically-stable, restrictive threshold = Hgb < 7g/dL
      • Orthopaedic surgery, cardiac surgery, or patients with CV = Hgb < 8g/dL
  • Complications
    • Hypocalcemia
    • Transfusion reactions
    • Alloimmunization

Fresh Frozen Plasma

  • Facts
    • 1 unit of FFP = 250cc
    • Contains all coagulation factors
    • Has an INR ~ 1.6
  • Storage Considerations
    • Frozen to -18-30oC within 8 hours of collection
      • Takes 10-30 minutes to thaw
    • Properly stored for up to 1 year
    • Must be used within 5 days after thawing
  • Indications for Transfusion
    • Vitamin K factor deficiency
      • Supratherapeutic vitamin K antagonist therapy
      • Liver disease
      • Massive transfusion protocol
    • DIC
    • TTP
  • Not indicated for hypovolemia and low BP
  • Complications
    • Male only plasma à decreases in TRALI


  • Facts
    • Precipitant that forms after FFP is frozen and thawed at 4oC
    • 1 unit of cryoprecipitate = 10-20cc
    • Contains fibrinogen, factor VIII, XIII, and vWF
  • Storage
    • Re-frozen at -18oC
      • Takes 10-30 minutes to thaw
    • Stored for up to 1 year
    • Only good for 4 hours once thawed and pooled, so order only when you need to transfuse
  • Indications for Transfusion
    • Low fibrinogen due to:
      • DIC
      • Liver disease
      • Uremia
      • Inherited disorders of fibrinogen
  • Complications
    • Same as other blood products


  • Facts
    • 2 collection types
      • Pooled
        • Centrifuged down from whole blood
        • Combined with other donors
      • Apheresis (single donor)
        • Platelets collected from donor and RBC and plasma returned
        • Allows matching
      • Volume ~300cc
      • Six-pack of pooled or one unit of apheresed platelet should raise platelet count by 30-60,000/uL
  • Storage
    • Stored at room temperature because cold induces clustering of vWF receptor
    • Only a 5-day shelf life
  • Indications for Transfusion
    • Actively bleeding patients with thrombocytopenia
    • Prevention of spontaneous bleeding
      • Varies depending on clinical condition
      • < 10,000/uL
      • No longer recommended to transfuse prophylactically when patient is on bypass – only is patient exhibits bleeding w/ thrombocytopenia or plt dysfunction
    • Preparation for invasive procedures:
      • Neurosurgery or ocular surgery – 100,000/uL
      • Most major surgery – 50,000/uL
      • Central line placement – 20,000/uL
      • Lumbar puncture – 50,000/uL
      • Epidural anesthesia – 80,000/uL
  • Complications
    • Increased infection risk

Component Modification Techniques


  • Indicated for:
    • Chronically transfused patients
    • Solid organ or bone marrow transplant patients
    • Previous febrile non-hemolytic reaction


  • Irradiation inactivates lymphocytes
  • Decreased risk of Graft-versus-host disease in immunocompromised patients
  • Reduces shelf life to 28 days


  • Rinsing serum proteins away prior to transfusions
  • Indicated for severe or recurrent allergic reactions and IgA deficiency
  • Indicated if a patient (especially neonate) is hyperkalemic


  • Indicated for immunocompromised patients

Transfusion Related Complications


  1. American Red Cross. Blood Facts and Statistics.  Accessed 03/23/2017.
  2. Brown MR, Jennings PR. Avoiding overtransfusion: an update on risks and latest indications. JAAPA. 2012;25(8):42-5. [pubmed]
  3. Basu D, Kulkarni R. Overview of blood components and their preparation.  Indian J Anaesth.  2014;58(5):529-537. [pubmed]
  4. Carson JL, Guyatt G, Heddle NM. Clinical Practice Guidelines From the AABB: Red Blood Cell Transfusion Thresholds and Storage. JAMA. 2016;316(19):2025-2035. [pubmed]
  5. Life In The Fastlane. Fresh Frozen Plasma.  Accessed 03/23/2017.
  6. Roback JD, Caldwell S, Carson J. Evidence-based practice guidelines for plasma transfusion. Transfusion. 2010;50(6):1227-39. [pubmed]
  7. Kaufman RM, Djulbegovic B, Gernsheimer T. Platelet transfusion: a clinical practice guideline from the AABB. Annals of internal medicine. 2015;162(3):205-13. [pubmed]
  8. Sharma S, Sharma P, Tyler LN. Transfusion of blood and blood products: indications and complications. American family physician. 2011;83(6):719-24. [pubmed]
  9. Takpradit K. Rational use of Blood Components.  Accessed 03/23/2017.

PAINE #PANCE Pearl – Hematology/Oncology


What is the scoring system used to grade Hodgkin’s Lymphoma and what are the different stages?



The Ann Arbor staging system with Cotswolds modifications is the current staging system used for patients with Hodkin’s Lymphoma.  There has been a meeting a subsequent meeting in Lugano, Switzerland in 2014, but those recommendations are controversial and not universally used.  The Ann Arbor staging system has four stages based on:

  • The sites of lymph node involvement
  • Extent of systemic disease

Image result for ann arbor staging

Image result for ann arbor staging



  1. Lister TA, Crowther D, Sutcliffe SB. Report of a committee convened to discuss the evaluation and staging of patients with Hodgkin’s disease: Cotswolds meeting. Journal of Clinical Oncology. 1989;7(11):1630-6. [pubmed]
  2. Cheson BD, Fisher RI, Barrington SF. Recommendations for initial evaluation, staging, and response assessment of Hodgkin and non-Hodgkin lymphoma: the Lugano classification. Journal of Clinical Oncology. 2014;32(27):3059-68. [pubmed]